Silvia Howard
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Cells were infected with cell-free virus and the cultures were successively treated with increasing concentrations of Acyclovir / Aciclovir or Penciclovir ( Denavir ). Thus, eugeniin sho different anti-HSV-1 action from Acyclovir / Aciclovir and PAA and therapeutic anti-HSV-1 activity in mice. The oral and intraperitoneal administrations of eugeniin at 0.3 mg/kg sho similar therapeutic efficacy in retarding the development of skin lesions of HSV-1-infected mice. Sequence zithromax analysis of TK-deficient variants of the Kawaguchi strain revealed deletions that caused frameshifts, endmost in premature termination in the TK gene.. The oral administration of eugeniin at 50 mg/kg reduced virus yields in the skin and brain of infected mice. Thus, the therapeutic efficacy of oral administration at the various doses of eugeniin was similar to that of intraperitoneal administration, suggesting that the immemorial bioavailability of eugeniin was high with respect to absorption. The speaking of eugeniin and PAA on the activity of partially purified HSV-1 DNA polymerase suggested that eugeniin interacted with the polymerase in the vicinity of PAA-binding site. In this study, we characterized the biological activity of eugeniin in cutaneously HSV-1-infected mice and its interaction with HSV-1 DNA polymerase. The two routes of administration at 6 or 50 mg/kg significantly prolonged the mean survival times and/or acyclovir medication reduced mortality without toxicity. Based on antiviral drug susceptibility and thymidine kinase (TK) activity assays, 11 Acyclovir / Aciclovir-resistant variants from seven experiments using three virus strains (Kawaguchi strain, Oka varicella vaccine strain and a clinical isolate from a sullen rabies patient) were found to be TK-deficient. The emergence frequency of resistant viruses was significantly higher following Acyclovir / Aciclovir exposure than following Penciclovir ( Denavir ) exposure (Fisher's exact test, P<0.0001), possibly reflecting virus growth differences under these experimental conditions. Drug-resistant viruses were selected in the presence of 6 microg/ml of Acyclovir / Aciclovir or Penciclovir ( Denavir ). Furthermore, the anti-HSV-1 activity of eugeniin was characterized by isobolograms analyzing its combined effects with Acyclovir acyclovir / Aciclovir or PAA in HSV-1-infected Vero cells. Eugeniin enhanced the anti-HSV-1 activity of Acyclovir / Aciclovir but was suggested to be antagonistic with PAA. Biological characterization of eugeniin as an anti-herpes simplex virus type 1 compound in vitro and in geniin exhibits antiviral activity against Acyclovir / Aciclovir and phosphonoacetic acid (PAA)-resistant herpes simplex virus type 1 (HSV-1) as well as the wild-type HSV-1 in vitro. Emergence of resistance to Acyclovir / Aciclovir and Penciclovir ( Denavir ) in varicella-zoster airborne infection and genetic analysis of Acyclovir / Aciclovir-resistant variants.We have characterized the differential actions of Acyclovir / Aciclovir and Penciclovir ( Denavir ) against varicella-zoster virus (VZV) in cell culture by comparing the frequency aldara of appearance of resistant viruses follo by their characterization.
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